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KMID : 0545120230330040543
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 4 p.543 ~ p.551
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Antimicrobial and Cytotoxic Activity of Endophytic Fungi from Lagopsis supina
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Dekui Zhang
Weijian Sun
Kim Jin-Mi
Changbo Ji
Yang Zhou
Jingyi Sun
Yutong Tian
Yanling Li
Fengchun Zhao
Yuan Tian
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Abstract
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In this study, five endophytic fungi belonging to the Aspergillus and Alternaria genera were isolated from Lagopsis supina. The antimicrobial activity of all fungal fermented extracts against Staphylococcus and Fusarium graminearum was tested using the cup-plate method. Among them, Aspergillus ochraceus XZC-1 showed the best activity and was subsequently selected for large-scale fermentation and bioactivity-directed separation of the secondary metabolites. Four compounds, including 2-methoxy-6-methyl-1,4-benzoquinone (1), 3,5-dihydroxytoluene (2), oleic acid (3), and penicillic acid (4) were discovered. Here, compounds 1 and 4 displayed anti-fungal activity against F. graminearum, F. oxysporum, F. moniliforme, F. stratum, Botrytis cinerea, Magnaporthe oryzae, and Verticillium dahliae with diverse MIC values (128?512 μg/ml), which were close to that of the positive control antifungal, actidione (64?128 μg/ml). Additionally, compounds 1 and 4 also exhibited moderate antibacterial activity against S. aureus, Listeria monocytogenes, Escherichia coli, and Salmonella enterica, with low MIC values (8?64 μg/ml). Moreover, compounds 1 and 4 displayed selective cytotoxicity against cancer cell lines as compared with the normal fibroblast cells. Therefore, this study proposes that the endophytic fungi from L. supina can potentially produce bioactive molecules to be used as lead compounds in drugs or agricultural antibiotics.
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KEYWORD
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Endophytic fungi, Lagopsis supina, antimicrobial activity, cytotoxic activity
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